Cryoconservation of animal genetic resources

These guidelines describe, in logical chronological order, the process of establishing a programme for cryoconservation of animal genetic resources, starting with the process of confirming the decision to implement a cryoconservation programme. The task of organizing the institutions needed to meet the chosen conservation objectives is then described. This is followed by an overview of the types of germplasm that can be cryoconserved, and technical details regarding the physical plant required to set up a gene bank and the quantities of germplasm required to ensure the capture of sufficient genetic variability. Principles of cryopreservation are then explained from a biological point of view and cryopreservation procedures discussed for different species and tissue types. The main body of the guidelins concludes with sections on sanitary measures, data management, legal issues and capacity building. Appendices provide detailed protocols and lists of equipment and reagents for collection and cryopreservation of various tissues for a range of common livestock species.